sappβ swe (Cell Signaling Technology Inc)
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94
Structured Review
Cell Signaling Technology Inc
sappβ swe
Sappβ Swe, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sappβ swe/product/Cell Signaling Technology Inc
Average 94 stars, based on 22 article reviews
Sappβ Swe, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sappβ swe/product/Cell Signaling Technology Inc
Average 94 stars, based on 22 article reviews
sappβ swe - by Bioz Stars,
2026-05
94/100 stars
Images
1) Product Images from "Loss of Ataxin-1 Potentiates Alzheimer's Pathogenesis by Elevating Cerebral BACE1 Transcription"
Article Title: Loss of Ataxin-1 Potentiates Alzheimer's Pathogenesis by Elevating Cerebral BACE1 Transcription
Journal: Cell
doi: 10.1016/j.cell.2019.07.043
Figure Legend Snippet: (A) Allele frequencies of ATXN1 with different CAG repeat lengths in the probands of NIMH AD families and healthy individuals of CEPH families. (B) Western blot analysis of APP processing in the brains of 3 month-old WT (+/+), Ataxin-1 hetero-KO (+/−) and KO (−/−) mice. RIPA buffer-soluble lysates were used to detect Ataxin-1 (ATXN1), APP, APP-CTF, ADAM10, BACE1. TBS-soluble lysates for sAPPα and sAPPβ. Actin is a loading control. Arrow head, proADAM10; *, non-specific band. (C) Densitometric quantification of western blot results. WT, relative value of 100. Values are mean ± SEM. n = 5. *p < 0.05, **p < 0.01, t-test. (D) Immunohistochemical (left) and immunofluorescence (right) staining for BACE1. Ctx, cortex; Hip, hippocampus; Cbl, cerebellum; Str, striatum; BSt, brain stem. Bar = 500 μm. (E) Ataxin-1, BACE1, and APP levels in dissected brain regions. OB, olfactory bulb. (F) Densitometry of BACE1 levels. n = 3. n.s.: not significant. ***p < 0.001. (G) RT-qPCR analysis of Atxn1 and Bace1 mRNA expression in the whole brain. n = 4. **p < 0.01, ***p < 0.001 versus WT Atxn1 mRNA; ##p < 0.01 versus WT Bace1 mRNA. (H) Bace1 mRNA levels in dissected cortex and cerebellum. n = 4. (I) Representative real-time qPCR amplification graphs for cortical cDNA. RFU, relative fluorescence unit. See also Figure S1.
Techniques Used: Western Blot, Control, Immunohistochemical staining, Immunofluorescence, Staining, Quantitative RT-PCR, Expressing, Amplification, Fluorescence
Figure Legend Snippet: (A) Actinomycin D or vehicle (DMSO) was treated for 48 hrs in acute brain slice cultures. Results are from two independent brain slice cultures (2 mice each for WT and Ataxin-1 KO). n (brain slices) = 3–4. *p < 0.05, **p < 0.01, ***p < 0.001, t-test. (B) Left: extracellular field potential records of multiple unit activity in CA1 areas of brain slices 48 hrs after incubation. Right: histograms representing spike amplitude distribution (bin size 10 mV) and inter-spike interval (bin size 2 ms). (C) Representative real-time qPCR amplification graphs of steady-state (total) and nascent Bace1 mRNA. Brain slices were incubated for 16 hrs in ACSF containing 5-ethynyl uridine. n (brain slices) = 4. (D) Locations of predicted CIC and PEA3 (ETV1/4/5) binding sites in Etv1/4/5 and Bace1 promoter regions, respectively. The CIC binding site in Etv1 promoter is relative distal (−1486) than those in Etv4/5. (E) CIC expression in medial cortex (left) and hippocampal CA1 (right). Bar = 50 μm. (F) mRNA levels of Etv1, Etv4, and Etv5 in the cortex and cerebellum. Gapdh was used as an internal control for RT-qPCR.All levels were presented as relative to Etv1-WT (100). Compared to cortex, the relative levels of Etv4 and Etv5 versus Etv1 mRNA are very low in cerebellum. n= 6 (cortex), 4 (cerebellum). (G) Western blot analysis for cortical levels of Ataxin-1, CIC, ETV4, ETV5, BACE1, sAPPβ, and sAPPα. (H) Cerebellar levels of ETV4 and ETV5. Vertical lines indicate that different parts within same blot were placed together for better comparison. (I) Densitometric quantification. n = 5, except ETV4 and ETV5 (n = 7). (J) Luciferase activity in HEK293T cells measured 24 hr after transfection with pGL3 plasmids harboring BACE1 promoter and vectors expressing either ETV4 or ETV5. Results from 3 independent experiments of sextuplicate transfection were normalized and combined. n = 18. See also Figure S2.
Techniques Used: Slice Preparation, Activity Assay, Incubation, Amplification, Binding Assay, Expressing, Control, Quantitative RT-PCR, Western Blot, Comparison, Luciferase, Transfection
Figure Legend Snippet: (A) Analysis of Ataxin-1, BACE1, ADAM10, sAPPα, sAPPβ, and sAPP levels in AD mouse brains. sAPPα and sAPPβ derived from either transgenic APPswe or endogenous APP were detected by different antibodies. (B) Densitometric quantification. Number of mice analyzed, n = 11–12. ***p < 0.001, t-test. (C) Ratio of transgenic sAPPα and sAPPβ. **p < 0.01. (D-E) Levels of APP-CTFα and - CTFβ in brains. Number in parenthesis denotes the number of mice analyzed. *p < 0.05. (F) TBS-insoluble Aβ40 and Aβ42 amounts in the brains of 5-month-old mice. The amounts of TBS-insoluble Aβ at this age were ~ 20 times higher than those of TBS-soluble Aβ. (G) Western blot analysis of detergent-soluble brain lysates with 6E10 antibody targeting N-terminal region of human Aβ. *, non-specific bands. (H) Densitometric quantification of Aβ levels. See also Figure S3.
Techniques Used: Derivative Assay, Transgenic Assay, Western Blot
Figure Legend Snippet: KEY RESOURCES TABLE
Techniques Used: Recombinant, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Luciferase, Reporter Assay, Staining, Expressing, Knock-In, SYBR Green Assay, Cloning, Mutagenesis, Software, Microscopy, Real-time Polymerase Chain Reaction, Transfection